western blot assay meaning in English
西式吸印杂交试验
Examples
- On one hand , antibody is necessary to western blot assay . the protein can be used to generate antibody
使用得到的蛋白来生产抗体用于下一步表达检测的western印迹试验。 - Western blot assay using purified chloroplast confirmed that only cp was existed in it
通过叶绿体的western - blot检测亦证明病毒粒子在叶绿体中的存在，而vp37蛋白在叶绿体中分布较少。 - Western blotting assay showed that product expression in larvae of silkworm was three proteins , and weights were 17kd , 20kd , and 29kd . because quantity of expression was n ' t enhanced , the co - expression of methology in this study is not desirable
4 、合前后表达产物的eusa测定和生物活性测定比较，融合后产物在表达量增加10 %情况下，生物活性并升高，推测可能与hgm一csf的空间结构有关。 - The expression activity reached the highest point at the 72nd hour in bmn cell ( 22 u / 2x ! 06cells ) and at 144th hour in larvae ( 159 u / ml ) , respectively . elisa assay showed that expression product had angiostatin ' s immunoreactivity . western blotting assay also showed that product expressed in cultured cells was a 36 kd band , while product expressed in larvae of silkworms was two proteins , and the molecular weights were 37 kd and 42 kd , respectively
表达产物活性在家蚕细胞中表达72小时达到最高值，经胞内表达产物作用后，血管内皮细胞的存活率仅为( 28 . 0 3 . 0 ) ，表达量约为22u 2 10 ~ 6个细胞；在家蚕体内表达144小时活性达到最高值，内皮细胞的存活率仅为( 6 . 4 0 . 5 ) ，表达量约159u ml 。 - Was first processed by dgd embedment and embedment - free technique and general technique for em morphology . a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed . then using western blot assay , we found a lamin - like component band of 68kd protein in the three - step - fractionated cells . to investigate the distribution of the lamin - like protein in cells , a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes . the results revealed that the positive reactivity presented at different part of the cells . the perinuclear cross - actions were distinct , and cross - action with the oral apparatus and the cortex were also obtained
本文以dgd包埋去包埋技术对草履虫的核纤层通过透射电镜和免疫荧光分子杂交等技术进行了观察。结果显示，在核周存在由10nm纤维组成的核纤层免疫荧光结果表明，在核周呈阳性反应，并在其表皮口器等部位呈交叉的阳性反应蛋白分子杂交的反应带在68kd处呈阳性反应。